5 Simple Techniques For 가슴수술
5 Simple Techniques For 가슴수술
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It can be critical that the submerged enlargement cultures in inserts reach a hundred% confluence in advance of air-lifting. At one hundred% confluence, the cells will address the area through the insert forming a whole, uniform monolayer.
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Employing a P1000 pipette, gently triturate BLOs from the apical chamber roughly three situations even though circling the idea around the insert. Transfer contents to your 15 mL conical tube.
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Observe: Carry on to antibody staining if performing Reside cell Examination immediately after this move. Alternatively, progress to fixable dye staining and PFA fixation.
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Perform cell counts utilizing an automated cell counter. Notice: Very low viability is usually optimized by the length of collagenase or ACCUTASE™ incubation. It may also be optimized by minimizing the amount of triturations.
This could cause lower Stem cell modifying efficiencies. To make sure availability of enough nutrients for CD34+ HSPC growth, it is suggested to keep the lifestyle focus reduced than one x 106 cells/mL.
The following fluorochrome-conjugated antibodies and dyes are encouraged to aid Investigation of gene-edited CD34+ HSPCs: